Michael Pyne
Michael Pyne
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Coupling the CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia coli
ME Pyne, M Moo-Young, DA Chung, CP Chou
Applied and environmental microbiology 81 (15), 5103-5114, 2015
Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium
ME Pyne, MR Bruder, M Moo-Young, DA Chung, CP Chou
Scientific reports 6 (1), 25666, 2016
Biochemical and genetic engineering strategies to enhance hydrogen production in photosynthetic algae and cyanobacteria
K Srirangan, ME Pyne, CP Chou
Bioresource technology 102 (18), 8589-8604, 2011
Engineering plant secondary metabolism in microbial systems
ME Pyne, L Narcross, VJJ Martin
Plant physiology 179 (3), 844-861, 2019
A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
ME Pyne, K Kevvai, PS Grewal, L Narcross, B Choi, L Bourgeois, ...
Nature Communications 11 (1), 3337, 2020
Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum
ME Pyne, M Moo-Young, DA Chung, CP Chou
Biotechnology for biofuels 6, 1-20, 2013
Technical guide for genetic advancement of underdeveloped and intractable Clostridium
ME Pyne, M Bruder, M Moo-Young, DA Chung, CP Chou
Biotechnology advances 32 (3), 623-641, 2014
Extending CRISPR-Cas9 technology from genome editing to transcriptional engineering in the genus Clostridium
MR Bruder, ME Pyne, M Moo-Young, DA Chung, CP Chou
Applied and environmental microbiology 82 (20), 6109-6119, 2016
A highly characterized synthetic landing pad system for precise multicopy gene integration in yeast
L Bourgeois, ME Pyne, VJJ Martin
ACS Synthetic Biology 7 (11), 2675-2685, 2018
Biochemical, genetic, and metabolic engineering strategies to enhance coproduction of 1-propanol and ethanol in engineered Escherichia coli
K Srirangan, X Liu, A Westbrook, L Akawi, ME Pyne, M Moo-Young, ...
Applied microbiology and biotechnology 98, 9499-9515, 2014
An Engineered Aro1 Protein Degradation Approach for Increased cis,cis-Muconic Acid Biosynthesis in Saccharomyces cerevisiae
ME Pyne, L Narcross, M Melgar, K Kevvai, S Mookerjee, GB Leite, ...
Applied and environmental microbiology 84 (17), e01095-18, 2018
Disruption of the reductive 1, 3-propanediol pathway triggers production of 1, 2-propanediol for sustained glycerol fermentation by Clostridium pasteurianum
ME Pyne, S Sokolenko, X Liu, K Srirangan, MR Bruder, MG Aucoin, ...
Applied and environmental microbiology 82 (17), 5375-5388, 2016
Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme
ME Pyne, M Moo-Young, DA Chung, CP Chou
Biotechnology for biofuels 7, 1-15, 2014
Developing an Extended Genomic Engineering Approach Based on Recombineering to Knock-in Heterologous Genes to Escherichia coli Genome
K Sukhija, M Pyne, S Ali, V Orr, D Abedi, M Moo-Young, CP Chou
Molecular biotechnology 51, 109-118, 2012
Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum
ME Pyne, X Liu, M Moo-Young, DA Chung, CP Chou
Scientific Reports 6 (1), 26228, 2016
Using the Endogenous CRISPR-Cas System of Heliobacterium modesticaldum To Delete the Photochemical Reaction Center Core Subunit Gene
PL Baker, GS Orf, K Kevershan, ME Pyne, T Bicer, KE Redding
Applied and environmental microbiology 85 (23), e01644-19, 2019
Antisense-RNA-Mediated Gene Downregulation in Clostridium pasteurianum
ME Pyne, M Moo-Young, DA Chung, CP Chou
Fermentation 1 (1), 113-126, 2015
Reconstituting plant secondary metabolism in Saccharomyces cerevisiae for production of high-value benzylisoquinoline alkaloids
ME Pyne, L Narcross, E Fossati, L Bourgeois, E Burton, ND Gold, ...
Methods in enzymology 575, 195-224, 2016
Improved draft genome sequence of Clostridium pasteurianum strain ATCC 6013 (DSM 525) using a hybrid next-generation sequencing approach
ME Pyne, S Utturkar, SD Brown, M Moo-Young, DA Chung, CP Chou
Genome announcements 2 (4), 10.1128/genomea. 00790-14, 2014
Enzyme biocatalysis
D Abedi, L Zhang, M Pyne, CP Chou
Elsevier, 2019
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